Isolation and Identification Rust Pathogens and the Study of Antioxidant Enzyme Activity and Gene Expression under Rust Infection in Zoysia japonica

The goal of this study was to identify the zoysiagrass rust pathogens and analyze differences in rust-resistant rust-susceptible Zoysia japonica germplasm upon inoculation. Based on assessment spore morphology 18S ribosomal DNA (rDNA) molecular identification, pathogen identified as Puccinia zoysiae Diet. development mycelium, rate spreading, timing production were more delayed (RR) genotype than (RS) genotype. After inoculation, activities superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) initially decreased, then increased both RR RS genotypes, but enzyme faster Rust resistance positively correlated with antioxidant activity. observed changes CAT, POD APX activity corresponded their gene expression levels. results may be utilized accurately evaluating damage disease rust-resistance aimed at breeding varieties improving management turf.